Fig. 4.

OGN reversed EMT through EGFR/Akt signal pathway. a HT29, SW620 cells with or without OGN over-expression were treated with EGF (100 ng/mL) for the indicated intervals. Cell lysates were subjected to Western blotting with the indicated antibodies referred to the three main downstream signals. b Cells with OGN over-expression were treated with EGF (100 ng/mL) and co-cultured with or without MG132 (1 μM) for 24 h. c Indicated cells were treated with EGF (100 ng/mL) for 15 min. Western blotting with the indicated antibodies regarding to EMT was performed. d Cells with OGN over-expression were challenged with EGF (100 ng/mL) and pretreated with SC79 (constitutive Akt activator) for 24 h. Western blotting with the significantly altered markers was performed