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. 2018 Mar 2;4:1. doi: 10.1186/s40738-018-0046-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Gating strategy based on pan lymphocyte marker CD45+, with positive staining for markers CD3 and CD8 and negative gating to acquire the CD4+ T-lymphocytes. Intracellular cytokine expression is then measured in the CD4+ cells. This strategy is required as CD4+ surface marker expression is suppressed by the stimulatory process