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. 2018 Mar 2;4:1. doi: 10.1186/s40738-018-0046-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Top panel: Flow cytometry report showing unstimulated CD3+ CD8- (CD4+) T-lymphocytes. Correct gating on these cells allows for accurate positioning of the corresponding stimulated cell profile on middle panel. Thus the ratio of TNFa and IFNg expression to IL-10 expression can be calculated. In this case IL-10: TNFa is 1:43 and IL-10 to IFNg is 1:22. The lower panel indicates a typical response seen in a nutrient “responder” with an increase in IL-10 and a decrease in levels of response to TNFa and IFNg