Fig. 3.

In vivo toxicity investigation. a Body weight changes, Liver index (liver weight/body weight, g/g). b Glutathione (GSH) and Superoxide dismutase (SOD) assay. c Biochemical indices (Alanine aminotransferase [ALT], Aspartate aminotransferase [AST], Total bilirubin [BIL], Creatinine [CRE], and Ureal nitrogen [URE]), and TNF-α levels. The four groups for (a, b, c) were as follows: PBS, Lp, Lp/pcDNA, and ILp/pcDNA group with POPC concentration of 10 mg/kg. Every four days, for a total of four doses with 200 μL of solution for each mouse, the mice were injected in the tail vein. The mice were sacrificed at 5 (one injection) and 17 (four injections) days post administration. Data are presented as the mean ± SD (n = 6); ^*P < 0.05 is considered statistically significant