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Effect of Pam3CSK4 on LPS-driven GLT expression. The resting B cells were stimulated with LPS (5 µg/ml) and Pam3CSK4 (0.5 µg/ml). After 3 days of culture, RNAs were isolated, and the levels of GLTs and AID mRNA were measured by RT-PCR (upper panel). cDNA from each sample were prepared at 1/5 and 1/25 dilutions, and the expression levels of GLTs were measured by PCR (lower panel). Data were obtained from 2 independent experiments (Exp1 and Exp2).
