Figure 6.

Effects of LPS and Pam3CSK4 on plasma cell differentiation. Resting B cells were stimulated with LPS (5 µg/ml) and Pam3CSK4 (0.5 µg/ml). (A) After 2.5 days of culture, RNAs were isolated and the levels of indicated mRNAs measured by RT-PCR. (B, C) Proportions of CD138⁺ (Syndecan-1⁺) B220^lo plasma cells. After 3 and 4 days of culture, harvested cells were stained with anti-CD138 PE and anti-B220 FITC Abs and analyzed by FACSCalibur. (B) Data shown are representative (3 days culture) of 2 independent experiments. (C) Data are averages of 3 and 4 days cultures from 2 independent experiments with SEM error bars.

SEM, standard error of the mean.