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. 2018 Jan 28;2018:7318513. doi: 10.1155/2018/7318513

Figure 2.

Figure 2

Ad-MSC increased proliferation of ESCendo and ESCcyst. A transwell system using Ad-MSC and conditioned medium (Cond) from allogeneic unprimed and primed (100 U/mL IFN-γ or 100 U/mL IFN-γ + 10 ng/mL TNF-α) Ad-MSC were used in coculture with ESCendo or ESCcyst. Direct cell coculture of unprimed Ad-MSC and ESCendo or ESCcyst was also carried out. Proliferation of ESCendo and ESCcyst was determined using the eosin exclusion (a), CFSE (b), and MTT (c) assays. Conditioned medium increased proliferation of ESCendo and ESCcyst (∗P < 0.05), and the transwell system had no effect on the proliferation of both cell types. The effect of direct cell coculture was more ambiguous. The data was normalized to untreated controls for each cell type. Thirty-six independent experiments (n = 3-4 biological replicates) were carried out in duplicates (mean ± SD).