Figure 3.
Ad-MSC reduced apoptosis and increased survival of ESCendo and ESCcyst. A transwell system using Ad-MSC and conditioned medium (Cond) from allogeneic unprimed Ad-MSC were used in coculture with ESCendo or ESCcyst. Direct cell coculture of unprimed Ad-MSC and ESCendo or ESCcyst was also carried out. Apoptosis and survival of ESCendo and ESCcyst was determined using the annexin V assay by flow cytometry. All three systems reduced apoptosis and increased survival of ESCendo and ESCcyst (∗P < 0.05). The data was normalized to untreated controls for each cell type. Four independent experiments (n = 4 biological replicates) were carried out in triplicates (mean ± SD).