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. 2018 Jan 28;2018:7318513. doi: 10.1155/2018/7318513

Figure 5.

Figure 5

Conditioned medium from Ad-MSC/ESCendo and Ad-MSC/ESCcyst cocultures increased tube formation of HUVEC on matrigel. HUVEC were cultured on matrigel for 17-18 hours in conditioned medium (Cond) from cell cocultures of allogeneic Ad-MSC/ESCendo or Ad-MSC/ESCcyst, and tube formation was determined. All conditions induced tube formation (a) (∗P < 0.05). Representative images showing connections between HUVEC forming ring or tube structures for each condition for ESCendo (b) and for ESCcyst (c), respectively. The images are at 4x magnification to image and quantify the whole 96-plate well. The data was normalized to untreated controls for each cell type. Four independent experiments (n = 4 biological replicates) were carried out in duplicates (mean ± SD). Scale bars represent 50 μm at 4x magnification.