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. 2018 Jan 28;2018:7318513. doi: 10.1155/2018/7318513

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Copyright © 2018 Fawaz Abomaray et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ad-MSC may promote migration of ESC_cyst. A transwell system using Ad-MSC and conditioned medium (Cond) from allogeneic Ad-MSC were used in coculture with ESC_endo or ESC_cyst. Following cell coculture, migration of ESC_endo and ESC_cyst was determined after 20 hours using the transwell migration assay. Conditioned medium reduced migration of ESC_endo (^∗P < 0.05). The transwell system increased migration, and conditioned medium reduced migration of ESC_cyst (^∗P < 0.05). Representative images of migration for each condition for ESC_endo (b) and ESC_cyst (c). The 0.4 μm pores of the inserts are the white dots, and the migrated cells are light/dark red in color (10x magnification). The data was normalized to untreated controls for each cell type. Three independent experiments (n = 3 biological replicates) were carried out in duplicates (mean ± SD).