Figure 7.

Ad-MSC did not increase invasion of ESC_endo and ESC_cyst. A transwell system using Ad-MSC and conditioned medium (Cond) from allogeneic Ad-MSC were used in coculture with ESC_endo or ESC_cyst. Following cell coculture, invasion of ESC_endo and ESC_cyst was determined using the transwell invasion assay after 20 hours. Conditioned medium reduced invasion of ESC_endo and ESC_cyst (^∗P < 0.05). Representative images of invasion for each condition for ESC_endo (b) and ESC_cyst (c). The 0.4 μm pores of the inserts are the white dots, and the invaded cells are light/dark red in color (10x magnification). The data was normalized to untreated controls for each cell type. Three independent experiments (n = 3 biological replicates) were carried out in duplicates (mean ± SD).