Figure 3.

MTDH is a target of miR-30a. A, Schematic representation of the miR-30a targeting sequences with the 3′-UTR of MTDH. Mutant 3′-UTR of MTDH were showed in red. B, A dual-luciferase reporter assay was performed in SW620 cells. Experiments were performed in triplicate. C, The protein level of MTDH was detected by Western blot in SW620 cells that were transfected with miR-30a mimics or negative control. GAPDH was included as an internal control. D, Expression of MTDH mRNA was detected by qRT-PCR. **P < .01 compared with the control. GAPDH indicates glyceraldehyde 3-phosphate dehydrogenase; miR-30a, MicroRNA-30a; mRNA, messenger RNA; MTDH, metadherin; UTR, untranslated region.