FIG. 4.

Histological evaluation. Micrographs of safranin O (saf O) and collagen type I, type II, and type X, immunostained histological cross-sections of native rabbit and TEC. In immunostained images, blue and green coloring is a result of a fast green counterstain, and darker coloring (red, brown, purple) is the result of the peroxidase reaction, indicating detection of the specific collagen type. B, bone; C, cartilage; GP, growth plate; PC, perichondrium; TEC, tissue-engineered cartilage; TM, tide mark. Color images available online at www.liebertpub.com/tea