FIG. 7.

The effect of T4 on types of collagen in neocartilage. (A) Coomassie blue-stained sodium dodecyl sulfate polyacrylamide gel electrophoresis showing the presence of type II and type XI collagen in pepsin extracts of TEC. Bovine type II collagen was electrophoresed as a control. (B) Densitometric semiquantitative analysis of type II and type XI collagen in TEC. AUC is the area under the pixel intensity plot curve. (C) Western blot probed with type X collagen antibody in 4 M GuHCl and pepsin extracts of TEC and native auricular cartilage as a positive control. In (A, C) the molecular weights of globular protein standards are shown. GuHCl, guanidine hydrochloride. Color images available online at www.liebertpub.com/tea