Table 1.
Gene | Primer sequence | Size (bps) | |
---|---|---|---|
Collagen X (COL10α1) | Forward | 5′-CCATCCTGGATCTCACAGAA-3′ | 115 |
Reverse | 5′-GCCACTAGGAATCCTGAGAA-3′ | ||
Collagen II (COL2α1) | Forward | 5′-GCTGGAGAAGAAGGCAAG-3′ | 391 |
Reverse | 5′-CAGGTTCACCATTGGCAC-3′ | ||
Collagen I (COL1α2) | Forward | 5′-CTCACCACCTTCTCTCAGAC-3′ | 298 |
Reverse | 5′-GGCCTTGGAGCTCTTATAC-3′ | ||
Superficial zone protein (PRG4) | Forward | 5′-CCACATCACCACCATCTTC-3′ | 344 |
Reverse | 5′-GCCACTTCCAGCTTCATC-3′ | ||
Biglycan (BGN) | Forward | 5′-AAGAACCACCTGGTGGAGA-3′ | 209 |
Reverse | 5′-GAGATGCGCAGGTAGTTGA-3′ | ||
18S | Forward | 5′-CACATCCAAGGAAGGCAG-3′ | 145 |
Reverse | 5′-CCTCCAATGGATCCTCGT-3′ |