FIG. 4.

Immunostained sections of 3D-engineered bone tissues cultured in normoxia (right) or hypoxia (left). Intensity profiles (center) were quantitated through each tissue along the indicated yellow arrow, starting from the interior of the tissue to the top surface. Parallel tissue sections were separately stained and quantitated for the presence of (A–D) ALP, (E–H) sclerostin, or (I–L) HIF1α. Negative controls were stained with (M) Isotype and (N) Secondary antibody only. All sections were counterstained with DAPI. Scale bar = 50 μm. ALP, alkaline phosphatase. Color images available online at www.liebertpub.com/tea