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. 2018 Feb 16;14(2):e1006876. doi: 10.1371/journal.ppat.1006876

Fig 9. Rhodomyrtone acts directly on the lipid bilayer without specific head group preference.

Fig 9

(A) Minimal inhibitory concentrations (μg/ml) of rhodomyrtone against different lipid head group mutants of B. subtilis. Daptomycin was used as a control. (B) Laurdan GP spectroscopy of liposomes prepared from E. coli polar lipid extract exposed to different concentrations of rhodomyrtone. Compound to lipid ratios: 1 μg/ml = 1:400, 10 μg/ml = 1:40, 50 μg/ml = 1:7. (C) Laurdan spectroscopy of liposomes treated with the membrane fluidizer benzyl alcohol. Compound to lipid ratios: 10 mM = 12.5:1, 30 mM = 37.5:1, 50 mM = 62.5:1. (D) SIM microscopy images of liposomes prepared from E. coli polar lipid extract exposed to different concentrations of rhodomyrtone for 5 min. Liposomes were stained with DiIC12. Compound to lipid ratios as in (B). Upper and lower rows show two examples from the same samples. Scale bar 1 μm. (E) Diameter of liposomes from (D i) and (D ii). Black bars represent mean with SEM.