Table 2.

Primers used for PCR amplification

Fragment	Primer	Sequence（5ʹ–3ʹ）	Target	Position
Universal primers (Simon et al. 2006)
F1	TK-J3790	CATTAgATgACTgAAAgCAAgTA	tRNA Lys	3,703–3,725
F1	A6-N4552	ATgTCCWgCAATYATATTWgC	ATP6	4,481–4,501
F2	C3-J4792	gTTgATTATAgACCWTgRCC	COIII	4,695–4,715
F2	C3-N5460	TCAACAAAATgTCARTAYCA	COIII	5,389–5,408
F3	N5-J7572	AAAgggAATTTgAgCTCTTTTWgT	ND5	7,395–7,418
F3	N4-N8727	AAATCTTTRATTgCTTATTCWTC	ND4	8,570–8,592
F4	CB-J10933	gTTCTACCTTgAggNCAAATRTC	Cytb	10,728–10,750
F4	CB-N11526	TTCTACTggTCgRgCTCCAATYCA	Cytb	11,343–11,363
Species specific primers (This study)
F5	Dp2-F	gAATgACTACAAAAACTTCCACCTgC	COI	2,887–2,912
F5	Dp3-R	TTAAggggTgATATTTgTgggATT	ATP8	3,845–3,868
F6	Dp4-F	AACCCTTgCTTTACCCTTATgATT	ATP6	4,303–4,326
F6	Dp5-R	ATTgAAAAACCTAATAGTAATAAA	COIII	4,794–4,817
F7	Dp6-F	CCTCgTTCTTTgTggCAACTggAT	COIII	5,255–5,278
F7	Dp7-R	TTAgggTTggTTTCTTATTgTTTggT	ND5	7,618–7,643
F8	Dp8-F	TAAACCAgAATAATAAgAACCAT	ND5	8,170–8,192
F8	Dp9-R	gCATTATCAACggCgAATCCTCC	Cytb	10,830–10,852
F9	Dp10-F	AATCCTATTTTgATCgTTggTAg	Cytb	11,300–11,322
F9	Dp11-R	AACTTTTTTTTCATTTTTACACTT	16SrRNA	12,875–12,898
F10	Dp12-F	TTCTCATCCAACCATTCATTCCAg	16SrRNA	13,071–13,094
F10	Dp13-R	AAAAggggAgATgggTTACAAT	12SrRNA	14,133–14,158
F11	Dp14-F	TTCAgAATAATAgggTATCTAATCC	12SrRNA	14,380–14,404
F11	Dp1-R	TCCTCTTTCTTgTCTgATAATgTggg	COI	2,174–2,199
F12	Dp15-F	ggAggTggAgATCCTATTCTAT	COI	2,080–2,101
F12	Dp16-R	ggACgCTCTATCTTggAgTAAT	COII	3,039–3,060
F13	Dp17-F	AATCCTATTTTgATCgTTggTAg	Cytb	11,300–11,322
F13	Dp18-R	TATATATTTTTgTTTATgggggTA	16SrRNA	13,264–1,3287
F14	Dp19-F	TgTTACgACTTATTTCACCTTggg	12SrRNA	13,986–14,009
F14	Dp20-R	gTTTgTgCCAgCAGTTgCggTTA	12SrRNA	145,49–14,571
For amplification and sequencing of the A+T-rich region
	Dp21-F	TAAAAAGAAGATCAAAATCCCAG	AT-rich	14,977–14,999
	Dp23-R	CTATAAgTTATTAAAAACTAAT	AT-rich	15,566–15,587
	Dp24-F	ATTAgTTTTTAAAAACTTATAg	AT-rich	15,566–15,587
	Dp22-R	TTgATACTTTAggAggTggTTT	tRNA Gln	85–106
	N = [A, C, G, T]; R = [A, G]; Y = [C, T]; W = [A, T]