Skip to main content
. 2018 Jan 23;9(2):78. doi: 10.1038/s41419-017-0103-7

Fig. 3. miR-374a directly regulates CCND1 in A549 and pc-9 cells or PTEN in SPCA-1 and H1975 respectively.

Fig. 3

a miR-374a and its putative binding sequences in the 3′UTRs of CCND1 and PTEN. Mutations were generated in the complementary site that binds to miR-374a. b, c. Western blotting detected protein levels of CCND1 and PTEN in miR-374a-overexpressing or miR-374a-inhibited NSCLC cells, with β-actin serving as a loading control. d Luciferase reporter assays were used to determine whether miR-374a directly targets the 3′UTR of CCND1 in A549 and pc-9 cells and PTEN in SPCA-1 and H1975 cells. Mean ± SD (n = 3). *P < 0.05. e Expression of CCND1 and PTEN was evaluated using immunohistochemistry of NSCLC xenografts. Scale bars, 50 μm. f Protein levels of CCND1, p-PI3K, p-AKT, E-cadherin, N-cadherin, c-MYC, CDK4, and P21 were detected by western blot in A549 and pc-9 cells after transfection with NC or CCND1 siRNA. g Protein levels of PTEN, β-catenin, TCF4, N-cadherin, c-MYC, and P21 were detected by western blot in SPCA-1 and H1975 cells after transfection with NC or PTEN siRNA