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. 2018 Jan 23;9(2):76. doi: 10.1038/s41419-017-0046-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The percentages of apoptotic male germ cells in wild-type mice a and P63^(+/⁻⁾ mice b at 2 months old. The percentages of early apoptosis c and late apoptosis d in male germ cells of wild-type mice and P63^(+/⁻⁾ mice were calculated using Student’s t-test. * indicated statistically significant differences (p < 0.05). TUNEL assay demonstrated the TUNEL-positive cells (green fluorescence) in P63^(+/⁻⁾ mice e and wild-type mice f. Replacement the TdT enzyme with PBS was used as the negative control g. PI (red fluorescence) was used to label cell nuclei. Scale bars in e–g = 10 μm. The data in Fig. 4a–g were presented from three independent experiments