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. 2018 Jan 19;9(2):58. doi: 10.1038/s41419-017-0082-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a In vitro tube formation of ECs treated with rapamycin or si-Beclin-1 at 6 h. Scale bar = 20 μm. b–f In vitro tube formation of EPCs at 6 h and quantification of total tube length, covered areas, total branching points, total loops, and total nets were measured by analytical software Image-Pro Plus 6.0 (IPP). g The expression of angiogenesis-related proteins VEGF and AngII of ECs were detected by western blot. h, i The expression of angiogenesis-related genes PCNA and VEGF of ECs were detected by RT-PCR. (^NSP > 0.05, n = 3)