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. 2018 Feb 7;9(2):182. doi: 10.1038/s41419-017-0258-2

Fig. 2. miR-143-3p inhibits GBC cell proliferation and angiogenesis in vitro.

Fig. 2

a, b Endothelial tube formation was estimated following incubation of HMVECs with conditioned medium from GBC cells transfected with mimics or inhibitors. The number of branches was quantified (**P < 0.01, ***P < 0.001; Student’s t test). Scale bar, 100 µm. c, d Invasion of HMVECs through the Matrigel chambers after incubation with conditioned medium from miR-143-3p-overexpressing or miR-143-3p-inhibited GBC cells for 48 h. Scale bar, 100 µm. The number of invading cells was calculated and is depicted in the bar graph (**P < 0.01, ***P < 0.001; Student’s t test). e Cell growth rates over 5 days were determined with CCK-8 proliferation assays (**P < 0.01, ***P < 0.001)