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. 2018 Feb 7;9(2):182. doi: 10.1038/s41419-017-0258-2

Fig. 5. ITGA6 is a direct target gene of miR-143-3p.

Fig. 5

a Potential miR-143-3p targets predicted by the three miRNA target-prediction programmes (TargetScan, PicTar and miRDB). b The wild-type or mutant ITGA6 3′UTRs to determine the miR-143-3p binding site. c, d Overexpression of miR-143-3p attenuates ITGA6 protein and mRNA expression in NOZ and GBC-SD cells compared with the miRNA-NC group. Silencing of miR-143-3p promotes ITGA6 protein and mRNA expression in NOZ and GBC-SD cells compared with the miRNA-NC group. (*P < 0.05, **P < 0.01; Student’s t test). e The relative luciferase activity of the wild-type or mutant ITGA6 3′UTR in 293T cells after transfections with the miR-143-3p mimic or inhibitor and corresponding control (*P < 0.05, NS no significant). f Expression of ITGA6 in GBC cells infected with the miR-143-3p mimic or inhibitor and corresponding control was examined by immunofluorescence. The red signals represent ITGA6 staining. The nuclei were counterstained with DAPI