Skip to main content
. 2018 Feb 15;9(3):255. doi: 10.1038/s41419-017-0199-9

Fig. 2. Chiron99021 and Valproic acid inhibit formation of ApcMin/Min cysts.

Fig. 2

a Images of organoids derived from ApcMin/+ after one (left) and two (right) passages in ENR or ENR-CV. b Proportion (%) of total organoids exhibiting cyst morphology at the end of each passage in ENR and ENR-CV. c Genotyping of organoids at the end of each passage shows that ApcMin/+ organoids grown in ENR-CV maintain the wild-type Apc allele while only the mutant allele is detectable in ENR. d Equal numbers of wild-type and ApcMin/Min organoids were mixed, and the percentage of organoids growing as cysts was determined after successive passages. e Genotyping of the mixed populations of wild-type and ApcMin/Min organoids shown in (d) confirms the disappearance of the wild-type allele in ENR and its re-emergence in ENR-CV corresponding to the relative abundance of cysts in each case