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. 2018 Jan 22;9(2):73. doi: 10.1038/s41419-017-0109-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The distribution of wild-type and mutant forms of FasL between raft (4–6) and non-raft (9,10) fractions was assessed using corresponding antibodies. b Expression levels of FasL and its mutant forms in the specified time periods were estimated by immunoblot analysis with anti-FasL Abs. Survival of HeLa cells overexpressing FasL variants, incubated with tetracycline during the indicated intervals (chart). Cell viability was determined by the neutral-red uptake assay. Results are presented as mean ± s.e.m of three independent experiments (each in triplicate). c Caveolin-1 knockdown by siRNA in HeLa-pcDNA4/TO-FasL cells decreases FasL cytotoxic effect. HeLa-pcDNA4/TO-FasL cells were transfected with psiRNA-h7SK-caveolin or psiRNA-h7SK-scramble control. Cell lysates were stained with anti-FasL mAbs (upper panel) and anti-caveolin-1 Abs (lower panel). Survival of HeLa-pcDNA4/TO-FasL cells transfected with psiRNA-h7SK-caveolin or psiRNA-h7SK-scramble control (chart). Cell viability was determined by the neutral-red uptake method. Results are presented as mean ± s.e.m. of four experiments (each in triplicate)