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. 2018 Jan 22;9(2):73. doi: 10.1038/s41419-017-0109-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Lysates of non-induced (left upper panel), tetracycline-induced for 4 h HeLa-pcDNA4/TO-FasL cells (right upper panel), HeLa cells transfected with mutant forms of FasL-Δ21 (left lower panel), and form I (right lower panel) were separated by ultracentrifugation in a sucrose gradient. Distribution of raft-specific (flotillin-1, caveolin-1, GM1 ganglioside (GM)) and non-specific (transferrin receptor1 (TfR1)) markers as well as FasL was analyzed by immunoblotting. b Expression levels of FasL and its mutant forms in the specified time periods were estimated by immunoblot analysis with anti-FasL Abs. Survival of HeLa cells overexpressing wild-type and mutant forms (FasL-Δ21 and form I) of FasL, incubated with tetracycline for the times indicated (chart). Cell viability was determined by the neutral-red uptake assay. Results are presented as mean ± s.e.m. of four experiments (each in triplicate)