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. 2018 Feb 14;9(2):247. doi: 10.1038/s41419-018-0283-9

Fig. 3. Impaired sympathetic neurogenesis but increased neural crest and glial progenitors in Isl1 CKO embryos.

Fig. 3

a qPCR verification of upregulated genes characteristic of NC and glial progenitors at E11.5–12. Error bars represent the s.d., n = 3, two-tailed t-test. b–e Co-immunostaining of ISL1/Sox10, Tom/Sox10, and Phox2b/ISL1 at E10.5 and E11.5. Scale bar, 50 µm. f–h Co-immunostaining of Sox10 and Phox2b in Isl1 CKO and control sympathetic ganglia showing a significant decrease in the number of Phox2b+ neurons (h2), but a significant increase in the number of Sox10+ (h1) and Phox2b+/Sox10+ (h3) progenitors. Error bars represent the s.d., n = 4, p = 0.0135 (h1), p = 0.0109 (h2), p = 0.0134 (h3), two-tailed t-test. Scale bar, 50 µm. i qPCR verification of upregulation of a number of genes of Notch signaling pathway and BMP antagonists at E11.5–12. Error bars represent the s.d., n = 3, two-tailed t-test. * p < 0.05, or ** p < 0.01