Skip to main content
. 2018 Feb 14;9(2):247. doi: 10.1038/s41419-018-0283-9

Fig. 4. Impaired differentiation and axon growth of sympathetic neurons in Isl1 CKO embryos.

Fig. 4

a qPCR analysis showing downregulation of genes involved in transcription, epigenetic regulation, and RNA binding on sympathetic ganglia of Isl1 CKO and control embryos at E11.5–E12. Error bars represent the s.d., n = 3, two-tailed t-test. b–m Selected genes downregulated in Isl1 CKO sympathetic ganglia were verified by in situ hybridization (b–k) or immunostaining (l, m) in SCG at E11. Scale bar, 50 µm. n, o TH+ immunostaining showing the absence of sympathetic innervation of the salivary gland in Isl1 CKO mice compared with controls. Scale bar, 200 µm. p–r Sympathetic ganglion explants culture showing significantly reduced axon growth in Isl1 CKO mutants compared with controls. Error bars represent the s.d., n = 3, p = 0.0005, two-tailed t-test. Scale bar, 100 µm. s qPCR confirming downregulation of a number of genes involved in axon growth and neurotransmission. Error bars represent the s.d., n = 3, two-tailed t-test. * p < 0.05, or ** p < 0.01