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. 2018 Feb 14;9(2):247. doi: 10.1038/s41419-018-0283-9

Fig. 6. RNA-seq analyses reveal dysregulation of genes important for sympathetic neuron differentiation in Isl1 hypomorphic mutants.

Fig. 6

a Scatterplot illustrating the relative gene expression of polyA-selected RNA transcripts from RNA-seq comparison of wildtype control and Isl1 hypomorphic mutant sympathetic ganglia. Genes upregulated (641) or downregulated (526) (p < 0.05) in Isl1 hypomorphic sympathetic neurons are shown in red and green, respectively. b, c GO terms enriched for genes upregulated or downregulated in Isl1 hypomorphic sympathetic ganglia. d–f GSEA showing that gene sets representing neuron differentiation and axonogenesis are downregulated, whereas a gene set representing the cell cycle are significantly upregulated in Isl1 hypomorphic sympathetic ganglia. Normalized enrichment scores (NESs) and p-values are shown. g, h qPCR validation of selected genes involved in axon growth, neurotransmission and ion channels, and the cell cycle in sympathetic neurons of E14.5 control and Isl1 hypomorphic embryos. Error bars represent the s.d., n = 3, two-tailed t-test. * p < 0.05, or ** p < 0.01