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. 2018 Jan 24;9(2):102. doi: 10.1038/s41419-017-0123-3

Fig. 5. RA blocked transdifferentiation of neonatal rat cardiac fibroblasts (CFs) via AMPKα in vitro.

Fig. 5

a, b The relative mRNA levels of collagen I (Col I) and collagen III (Col III) in CFs (n = 3). c Statistical results on cell viability evaluated by cell count kit 8 (n = 5). d Representative western blots and statistical results (n = 6). e, f Representative immunofluorescence staining of α-SMA in the presence or absence of shAmpkα2 and statistical results (n = 6). Green represented α-SMA, nuclei was stained with DAPI (blue). g Representative images of wound scratch assay at 0 and 12 h (n = 3). Values represent the mean ± SEM of three independent experiments. *P < 0.05 vs. the corresponding PBS-treated group within shRNA-treated CFs, #P < 0.05 vs. TGF-β-treated CFs within shRNA group, &P < 0.05 vs. the corresponding PBS-treated CFs in shAmpkα2 group. In Figs. 5a, b and 5d, *P < 0.05 vs. the matched group, NS no significance