Fig. 5. HOXA13 expression was regulated positively by HOTTIP and correlated with SCLC prognosis.

a, b HOTTIP knockdown may reduce expression of HOXA13 mRNA and protein in H69AR and H446AR cells, whereas HOXA13 overexpression may rescue the HOXA13 suppression caused by HOTTIP knockdown. c, d Knockdown of HOXA13 was successful both at mRNA and protein levels in H69AR cell. e Correlation scatterplot (Spearman test) between HOTTIP and HOXA13 expression levels was detected in clinical samples. f, g The effect of HOXA13 on cell chemoresistance and proliferation were observed by CCK-8 assay and plate clone formation. h The relationship between HOXA13 expression and clinical pathological characteristics was detected by immunohistochemistry. i Survival differences between groups with different scores were statistically assessed by the Kaplan–Meier method and log-rank test. *P < 0.05; **P < 0.01