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. 2018 Jan 23;9(2):82. doi: 10.1038/s41419-017-0093-5

Fig. 7. p53 facilitates HIF-1α ubiquitination and consequent proteasomal degradation in an MDM2-dependent manner.

Fig. 7

a HIF-1α polyubiquitination examined by immunoprecipitation and western blot of primary cardiomyocytes in the presence of MG132 with or without PFT-α or p53-siRNA treatment. b The MDM2 protein expression detected by western blot analysis. c, d The protein-protein interaction between MDM2 and HIF-1α detected at 3 (c) and 6 (d) months after diabetes onset by immunoprecipitation in vivo. e The protein-protein interaction between MDM2 and HIF-1α detected by immunoprecipitation in primary cardiomyocytes after transfection with Ctrl-siRNA or MDM2-siRNA. f HIF-1α polyubiquitination examined by immunoprecipitation and western blot in primary cardiomyocytes after transfection with Ctrl-siRNA or MDM2-siRNA. Data expressed as mean ± SD of three independent experiments. *P < 0.05 vs. Ctrl; #P < 0.05 vs. DM