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. 2018 Jan 18;9(2):37. doi: 10.1038/s41419-017-0118-0

Fig. 2. M1 suppresses hepatocytes apoptosis and inflammatory cytokines production caused by GalN/LPS.

Fig. 2

C57BL/6J mice were intraperitoneal injected either with vehicle or 30 mg/kg M1 every 8 h one time for three times. Blood and liver tissues were collected at 4 h after GalN (400 mg/kg)/LPS (10 μg/kg) treatment. a Hepatocytes apoptosis were measured by TUNEL staining and the number of apoptotic cells were counted (n = 5). Scale bars: 50 μm. b The levels of cleaved caspase 3, caspase 8, caspase 9, and PARP were measured by western blot (n = 5). c The expression of hepatic genes Gadd45α, Gadd45β, and A20 were determined by QPCR (n = 3). d Serum TNFα and IL-1β levels were assayed using ELISA method (n = 8). e Hepatic TNFα and IL-1β mRNA expression were determined by QPCR (n = 4–6). f F4/80 staining of liver sections were conducted to indicate the filtration of macrophages. Scale bars: 50 μm. g Hepatic MPO activity was measured according to manufacturer’s instruction (n = 5–6). Each value is mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001