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. 2018 Jan 19;9(2):61. doi: 10.1038/s41419-017-0097-1

Fig. 3. HBX specifically inhibits CHK2 phosphorylation.

Fig. 3

a, b Western blotting for γH2AX in control and HBX-expressing SUDHL-4 a, and DB b, cells with or without MTX treatment (4 ng/ml) for 48 h. c, d Western blotting for DDR proteins (P-CHK2/CHK2/ATM and P-CHK1/CHK1) in control and HBX-expressing SUDHL-4 c, and DB d, cells with or without MTX treatment (4 ng/ml) for 48 h. e, f The mRNA levels of DDR proteins (ATM, CHK2, and CHK1) were largely unchanged in control and HBX-expressing SUDHL-4 e, and DB f, cells with or without MTX treatment. g, h The expression of the P-CHK2 downstream genes P53 and P21 in control and HBX-expressing SUDHL-4 g, and DB h, cells with or without MTX treatment. The results are shown as the mean ± SEM from triplicate experiments. GAPDH was used as a loading control