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. 2018 Feb 13;9(2):218. doi: 10.1038/s41419-018-0323-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a DIO-labeled H1299 cells (green) were incubated with CM-Dil-labeled hUCMSC-EVs (red) for 2 h, and the hUCMSC-EV uptake by the LUAD cells was determined. The arrows indicate the fusion of the membrane. The scale bars indicate 50 μm. b FACS analysis of H1299 cells that had been treated for 12 h with CM-Dil-labeled hUCMSC-EVs or serum-free medium as control. The H1299 cells were pre-stimulated with hUCMSC-EVs for 12 h before they were injected into the nude mice. c The tumor volumes were measured, and d the tumor weights were determined at 35 days. e Imaging of tumors and tumor-bearing mice from the hUCMSC-EV-pre-stimulated groups at 35 days after the injections. f Ki-67 and g TUNEL immunohistochemical staining on tumor sections from the control or hUCMSC-EV-pre-stimulated group. The images are shown at ×100 (left panel) and ×400 (right panel)