Fig. 3. TR₄ suppresses HCC cell migration and invasion.

a, b MTS proliferation assay demonstrates that knocking down TR₄ has little influence on LM3 and Huh7 cells proliferation. c, d MTS proliferation assay demonstrates that overexpression of TR₄ has little influence on LM3 and Huh7 cells proliferation. e, i Knocking down TR₄ promotes LM3 cells migration and invasion. f, j Knocking down TR₄ promotes Huh7 cells migration and invasion. g, k Overexpression of TR₄ suppresses LM3 cells migration and invasion. h, l Overexpression of TR₄ suppresses Huh7 cells migration and invasion. The migrated or invaded cells were stained with crystal violet (0.1%) and positively stained cell numbers in six randomly picked areas were averaged. Experiments are repeated three times and mean ± SD values are shown in quantification. P-values presented in figures, ***P < 0.001