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. 2018 Feb 15;9(3):283. doi: 10.1038/s41419-018-0287-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — The luc-LM3-scr and luc-LM3-shTR₄ cells (2 × 10⁶) were orthotopically injected into the left lateral lobe of the liver of athymic nude mice. a IVIS imaging was used to determine the tumor size and metastasis, and the results showed that no metastasis was detected in any of the five mice that were injected with luc-LM3-scr cells. In three of the five mice that were injected with luc-LM3-shTR₄ cells, tumor metastasis was observed on Day 42. b HE staining results of HCC and the metastatic tumor tissues. c IHC staining was used for detecting TR₄ and EphA2 expression levels in tumor tissues obtained from the two groups of mice. d RT-qPCR was used for detecting TR₄ and EphA2 expression levels in tumor tissues obtained from the two groups of mice.

Fig. 6 — The luc-LM3-scr and luc-LM3-shTR₄ cells (2 × 10⁶) were orthotopically injected into the left lateral lobe of the liver of athymic nude mice. a IVIS imaging was used to determine the tumor size and metastasis, and the results showed that no metastasis was detected in any of the five mice that were injected with luc-LM3-scr cells. In three of the five mice that were injected with luc-LM3-shTR₄ cells, tumor metastasis was observed on Day 42. b HE staining results of HCC and the metastatic tumor tissues. c IHC staining was used for detecting TR₄ and EphA2 expression levels in tumor tissues obtained from the two groups of mice. d RT-qPCR was used for detecting TR₄ and EphA2 expression levels in tumor tissues obtained from the two groups of mice.