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. 2018 Feb 12;9(2):207. doi: 10.1038/s41419-018-0279-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Immunofluorescence staining of CD29 and CD13 on hJBMMSCs. b Oil red staining indicated lipid restoration in hJBMMSCs after cultured in lipid-forming medium for 7 days. c Alkaline phosphatase (ALP) staining of hJBMMSCs cultured in regular medium (control) and osteogenic (OS) medium at day 7, 14, and 21. d Quantitative ALP activity in hJBMMSCs at day 7, 14, and 21. e Alizarin red staining of hJBMMSCs cultured in regular medium and OS at day 21 and 28. f Quantification of Alizarin red staining in hJBMMSCs. g Western blotting of ALP in hJBMMSCs cultured in regular medium and OS. h Western blotting of Runx2 and Osterix in hJBMMSCs cultured in regular medium and OS. i mRNA expression of ALP, Runx2, and Osterix in hJBMMSCs at day 1, 3, 7, and 14. (n = 6–10/group; *p < 0.05, **p < 0.01, ***p < 0.001)