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. 2018 Feb 14;9(2):233. doi: 10.1038/s41419-018-0275-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Expression alteration of FOXD2-AS1 was shown in FOXD2-AS1 overexpression bladder cancer cells. b qRT-PCR was used to evaluate expression of FOXD2-AS1 in two independent siRNAs transfected bladder cancer cells. The figures in A and B showed representative results of three independent experiments. c and d MTT assays were performed to determine the T24 and UM-UC-3 cell growth capacities after silencing of FOXD2-AS1 via specific siRNAs transfections. The representative figure of three independent experiments showed that FOXD2-AS1 obviously promoted bladder cancer cell growth. *p < 0.05, **p < 0.01. e FOXD2-AS1 increased colony formation of bladder cancer cells. Left: representative figure of colony formation assays. Right: Error bars represent the mean ± S.D. from three independent experiments. *p < 0.05, **p < 0.01. f The representative figure of EdU assays demonstrated that knockdown of FOXD2-AS1 decreased the number of cells in the S phase. Error bars represent the mean ± S.D. from three independent experiments. *p < 0.05, **p < 0.01. g and h Cell migration distances and invasiveness were also (b) positively associated with FOXD2-AS1 expression in T24 and UM-UC-3 cells. Error bars represent the mean ± S.D. from three independent experiments. *p < 0.05, **p < 0.01