Fig. 5. Geldanamycin decreases the protein level of c-FLIP and enhances TRAIL sensitivity.
a, b Geldanamycin (GA) promotes c-FLIPL degradation. AGS cells (a) or SNU-16 (b) cells were treated with GA at the indicated concentrations for 24 h. The levels of DTX1, c-FLIPL, and c-FLIPS were determined by immunoblotting. c GA sensitizes AGS cells to TRAIL-induced apoptosis. AGS cells were treated with 0.5 μM GA overnight and then incubated with TRAIL for 24 h. Cell viability was determined by MTT assay. Values are means ± standard deviations of three independent experiments. ***P ≤ 0.001