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. 2018 Feb 15;9(3):275. doi: 10.1038/s41419-018-0311-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — WISH showing impaired wnt3a-induced expression of scl and gata1a in wnt3a mRNA and cftr morpholino co-injected zebrafish (a) and decreased expression of cdx4 and hoxa9a in cftr mutants (b). c Quantitative RT-PCR showing significantly reduced c-myc and lef1 mRNA levels in cftr mutants. d CFTR knockdown by siRNA attenuated the Wnt reporter TopFlash activity in HEK293 cells (n = 3). e Western blot analysis showing reduced Dvl2, 3 and nuclear β-catenin expression in cftr mutants. f Quantitative RT-PCR showing no significant changes in dvl2, 3 mRNA levels in cftr mutants. g Western blot analysis showing increased Dvl1-3 protein levels in co-expressing Cftr with Dvl1-3 HEK293 cells as compared to Dvl1-3 alone controls. h Effects of dvl2 mRNA in rescuing hematopoietic defects in cftr mutants. Embryos shown in a, b, and h are dorsal views with anterior to the top at 8-somite stage (13 hpf)