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. 2018 Feb 14;9(2):234. doi: 10.1038/s41419-018-0318-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The sketches illustrating the EC culture of HH0 gastrula chick embryos in the absence/presence of Baicalin (see Materials and method section for details). b The representative bright-field images were taken from control (first row), 3 μM Baicalin (second row), 6 μM Baicalin (third row), 12 μM Baicalin (fourth row) and 24 μM Baicalin (fifth row) groups at incubation times of 0, 26, 39 and 48 h, respectively. b1–b4 Bar charts showing the comparison of chick embryo length at 26 h (b1), 39 h (b2), 48 h (b3) and somite pair numbers at 48 h (b4) in the absence/presence of various concentrations of Baicalin. c The representative bright-field images of normal (left) and abnormal (right) gastrula chick embryos after 48 h of incubation. c1 Bar chart showing the comparison of normal and abnormal chick embryo numbers in the absence/presence of various concentrations of Baicalin.*p<0.05 compared with control group; **p<0.01 compared with control group; ***p<0.001 compared with control group. Scale bars = 1000 µm in (b, c)