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. 2018 Feb 14;9(2):234. doi: 10.1038/s41419-018-0318-2

Fig. 5. The extent of apoptosis in the absence/presence of HG and Baicalin.

Fig. 5

a The western blot data showing the C-caspase-3 expression in HH10 chick embryos exposed with either simple saline (control) or HG or/and Baicalin. b Bar chart showing the comparison of CCK8 values of HUVECs incubated with/without HG or/and Baicalin. c The propidium iodide (PI) flow cytometric assay was implemented in 48-h incubated HUVECs from control, 6 μM Baicalin, 50 mM glucose and 6 μM Baicalin+50 mM glucose group. c1 The bar chart showing the comparison of the values of B2 (late apoptosis)+B4 (early apoptosis) in the cultured HUVECs among control, 6 μM Baicalin, 50 mM glucose and 6 μM Baicalin+50 mM glucose group. (d–g) The representative bright-field images of 48-h cultured HUVECs from control (d), 6 μM Baicalin (e), 50 mM glucose (f) and 6 μM Baicalin+50 mM glucose (g) group. d1–g1 The fluorescent staining of PI and Hoechst was implemented on 48-h cultured HUVECs as in (d–g), respectively. d2–g2 The fluorescent staining of F-actin and DAPI was implemented on 48-h cultured HUVECs as in (d–g), respectively. h Bar chart showing the cell surface area of HUVECs in the absence/presence of HG or/and Baicalin. i Bar chart showing the ratio comparison between PI+ and Hoechst+ HUVECs in the absence/presence of HG or/and Baicalin. *p<0.05 compared with control group; ***p<0.001 compared with control group; #p<0.05 compared with HG group; ##p<0.01 compared with HG group; ###p<0.001 compared with HG group. Scale bars = 100 µm in (d–g1) and 50 µm in (d2–g2)