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. 2018 Jan 24;9(2):89. doi: 10.1038/s41419-017-0159-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a, e A549 and NCI-H460 cells were incubated with various concentrations of AF with or without different concentrations of ADM for 72 h. Cell viability was determined by MTS assay. b, f Analysis of drug CI of AF and ADM in A549 and NCI-H460 cells. CI values were calculated using the Calcusyn software (Biosoft). c, g A549 and NCI-H460 cells were incubated with AF, ADM, or their combination. The numbers of cell colonies were counted after 14 days. d, h Comparison of the observed drug combination (AF + ADM) effect (third column) and the predicted additive effect (fourth column) on colony formation. The predicted additive viable cell percentage was calculated by multiplying the respective % of viable cells treated by either AF or ADM alone. Each bar represents mean ± SD of three separate measurements, **p < 0.01