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. 2018 Feb 20;9(3):296. doi: 10.1038/s41419-018-0342-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Expression of ER stress-related proteins in the two OSCC cell lines. β-Actin was used as loading standard. All protein bands were related to their respective control set to unity and corrected by the relative intensity of the loading control by using the densitometry function in ImageJ. b Immunofluorescence staining of calnexin in OSCC cell lines post 24 h of erufosine treatment. An increase in overall fluorescence intensity of calnexin in HN-5 and SCC-61 was seen (b, d) between control and treated cells. This was confirmed by CTCF (c, e), which was significantly increased in the two cell lines (p < 0.001). The CTCF was calculated as follows: (corrected total cell fluorescence) = integrated density − (area of selected cell × mean fluorescence of background readings). The experiment shows an average of three independent repeats.