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. 2018 Feb 22;9(3):307. doi: 10.1038/s41419-018-0348-9

Fig. 2. Knockdown of PIM1 suppresses the growth of ccRCC cells in vitro.

Fig. 2

a, b Edu assays. The cells were stained with Edu (green) and a nuclear dye, Hoechst (blue). Edu-positive cells were counted with ImageJ software, and the percentage of positive cells was calculated. Scale bar: 20 μm. c, d Colony formation assays. The number of colonies were quantified with ImageJ software. Data are shown as the mean ± S.E.M. of the values from triplicate experiments. Statistical significance was assessed with a two-tailed Student’s t-test. *P < 0.05; and **P < 0.01