Table 1.
Effect of 24 h of incubation of alveolar basal epithelial A549 and microglial BV-2 cells with engineered carbon nanodiamonds (ECNs) on adenine nucleotides (mono, di, and triphosphorylated), cell energy state (ECP) and mitochondrial phosphorylating capacity (ATP/ADP ratio)
| ATP (nmol/mg protein) | ADP (nmol/mg protein) | AMP (nmol/mg protein) | ECP | ATP/ADP | |
|---|---|---|---|---|---|
| Resting A549 | 95.07 (19.02) | 8.25 (2.03) | 1.24 (0.34) | 0.96 (0.11) | 11.52 (1.12) |
| A549 + ECNs | 72.73* (14.58) | 9.34** (1.18) | 4.19* (0.72) | 0.89* (0.12) | 7.79* (0.65) |
| Resting BV-2 | 123.90 (29.68) | 15.93 (2.35) | 2.07 (0.62) | 0.93 (0.07) | 7.78 (1.12) |
| BV-2 + ECNs | 87.87* (17.42) | 26.83* (5.65) | 8.66* (1.51) | 0.82* (0.11) | 3.28* (0.94) |
Values are the mean of four different experiments. Standard deviations are in parenthesis. Incubation conditions and HPLC method to separate simultaneously the indicated compounds are given in Materials and methods
ECNs engineered carbon nanodiamonds, ATP, ADP, AMP adenosine mono, di, triphosphate, respectively; ECP energy charge potential (ATP + 1/2 ADP/ATP + ADP + AMP)
*Significantly different from resting, p < 0.001; **Significantly different from resting, p < 0.05