Table 2.
Effect of 24 h of incubation of alveolar basal epithelial A549 and microglial BV-2 cells with engineered carbon nanodiamonds (ECNs) on purine and pyrimidine nucleotides (mono, di, and triphosphorylated)
| GTP (nmol/mg protein) | GDP (nmol/mg protein) | GMP (nmol/mg protein) | IMP (nmol/mg protein) | UTP (nmol/mg protein) | UDP (nmol/mg protein) | UMP (nmol/mg protein) | CTP (nmol/mg protein) | CDP (nmol/mg protein) | CMP (nmol/mg protein) | |
|---|---|---|---|---|---|---|---|---|---|---|
| Resting A549 | 25.71 (8.44) | 4.51 (1.13) | 0.64 (0.10) | 0.40 (0.05) | 39.56 (4.05) | 0.58 (0.09) | 0.18 (0.04) | 26.32 (3.47) | 0.52 (0.12) | 0.047 (0.018) |
| A549 + ECNs | 16.14* (2.07) | 5.69* (1.38) | 0.96* (0.12) | 0.49* (0.07) | 32.73* (3.71) | 1.20* (0.38) | 0.16* (0.03) | 20.10* (2.21) | 0.76* (0.17) | 0.043* (0.011) |
| Resting BV-2 | 21.79 (5.13) | 6.73 (2.02) | 2.05 (0.79) | 2.56 (0.31) | 45.83 (6.25) | 3.09 (0.89) | 0.77 (0.26) | 16.34 (3.32) | 2.45 (0.68) | 1.37 (0.45) |
| BV-2 + ECNs | 17.32* (3.37) | 6.01* (1.51) | 4.85* (0.74) | 2.28* (0.56) | 33.36* (8.13) | 4.58* (1.64) | 0.59* (0.28) | 10.92* (2.45) | 1.95* (0.72) | 1.32* (0.19) |
Values are the mean of four different experiments. Standard deviations are in parenthesis. Incubation conditions and HPLC method to separate simultaneously the indicated compounds are given in Materials and methods
ECNs engineered carbon nanodiamonds, GTP, GDP, GMP guanosine mono, di, triphosphate, respectively; IMP inosine monophosphate, UTP, UDP, UMP uridine mono, di, triphosphate, respectively; CTP, CDP, CMP cytidine mono, di, triphosphate, respectively
*Significantly different from resting, p < 0.01