Fig. 6. Knockout of PP5 promotes chondrogenic differentiation markers in articular cartilage.

Gene expression analysis of articular cartilage from WT and PP5 KO mice indicated that the ablation of PP5 resulted in an almost 90% reduction of PP5 mRNA expression (a). Several chondrogenic differentiation markers such as aggrecan (Acan), Col II, and Col X were significantly increased in the articular cartilage of PP5 KO mice (b–d). In addition, although mRNA expression of Col I was significantly decreased (e), the expression of Alp, Runx2, Opn, and Ocn were significantly increased in the PP5 KO mice (f–i). Values are presented as mean ± SE. *P < 0.05, **P < 0.01, and ***P < 0.005 using Student’s t-test. n = 3