Fig. 5. NFYA was a pro-oncogene in human Ishikawa and RL-95-2 cell lines.

a, b Auxo-effect of NFYA on the proliferation of Ishikawa and RL-95-2 cell lines, as depicted by CCK-8 and EdU assay results. c Representative flow cytometry results of the cell cycle phases of Ishikawa and RL-95-2 cell lines with transfection of NFYA are shown. d Representative flow cytometry findings of apoptotic Ishikawa and RL-95-2 cell lines are shown with transfection status of NFYA indicated. e Wound healing assays were carried out to investigate the cell migration capacity. f Transwell assays were performed to investigate the quantities of invading cells when NFYA expression was altered. Representative images and accompanying statistical plots are presented. Data are presented as the means ± SD from three independent experiments. *P < 0.05